Protein Synthesis

only rarely is there an opportunity to participate in a molecular happening you're going to have that opportunity for this film attempts to portray symbolically yet in a dynamic and Joyful Way one of Nature's fundamental processes the linking together of amino acids to form a protein we know now that the threedimensional structure and the function of a protein is determined by the order of amino acids along the backbone of the molecule so protein synthesis involves programming and assembly and this film with people portraying molecules using the dance idiom tries to animate these two processes the programming and assembly of a protein our genes carry the instructions for ordering the amino acids of each protein those instructions are encoded in a messenger molecule and RNA depicted in this film as a long snaking chain each of the message units is played by three adjacent people in the chain colored head balloons indicate the bases green for guanine blue for uracil yellow for adenine if there is a message there must be a way to translate that message and that's the job of the ribosome and of the transfer rnas the ribosome is composed of a large and of a small subunit and these are depicted in the film as tumbling rolling clusters of of body amorphous by themselves but organized and structured when in the act of translating the message first the small subunit with the aid of an initiation Factor captures the message then the first Transfer RNA carrying its cognate amino acid is brought to the ribosome message complex by a second dancing initiation Factor this requires energy and that's represented by a puff of smoke next the large ribosomal subunit tumbles into place and then the process of bringing each amino acid to the ribosome message complex is accomplished by the T factor and its GTP cohort and so in the order prescribed by the balloon colors in the message chain each amino acid is brought to the assembly site to be added to the growing Chain by the peptidal synthetase next in an energy requiring step the message RNA TRNA complex is shifted so as to bring the next message unit into the ribosome to allow the process to repeat itself at the end the Terminator Factor seeing the termination signal cleaves the completed protein from the last TRNA releasing it from the ribosome so that the ribosome can do its job again the two subunits are split apart and separated from the messenger by the ribosome releasing Factor my diagram is of necessity stat IC but protein synthesis is a dynamic process this movie tries to bring those Dynamic interactions to life [Applause] was brillig and the slidy 30s ribosome did guy and gimbal in the wave 30s ribosome [Music] [Applause] [Music] all Mimsy was mRNA that colored message unit array [Music] mRNA mRNA a [Music] bound they were in the Glorious Sun by initiator Factor 1 [Music] initia Factor 2 initiator Factor 2 went searching for TRNA Who Bore the flaccid amino [Music] acid t [Music] a g woo then as the eish bonds were formed the 50s ribosome Untamed came whiffling the tgy grass and burble as it [Music] came 50s rap Stone [Music] W [Music] wo [Music] wooo Tor Tor T Factor plus GTP gathered in TRNA T woo [Music] wow gathered into RNA and came gumping [Music] back hydrogen [Music] B amino acids both inside alas the peptide bond was formed [Music] and as the index step took place the message units shift a [Music] [Applause] [Music] space the [Music] t w t RNA a w [Music] each TRNA approached the site bearing its amino acid load whose sequence was determined by the MRNA message unit magic [Music] code peptide [Music] andex andex woo clipping clipping clipping clipping clipping inside inside [Music] have the Dan that [Music] oh long time did bio complex turn the protein grew by by tibs and TOS amino acids linked in turn by the catalytic [Music] [Applause] [Music] ribosome stop stop protein reling Factor protein reling protein releasing Factor since the end her voral arms went Snicker snout oh fra just day Koo Cay the protein chain came streaming [Music] out then ribosome releasing Factor was Jaws that bite and Claws that free released the synthesizing complex and chortled in his [Music] Glee Twi brillig in the slidy ribosome did guy and gimbal in the wave all Mimsy was mRNA and protein chain out Gra [Music] wo wo w [Music] w w w [Music]