Nuclear Transplantation

Reveals, through photomicrography and time-lapse sequences, the research technique of nuclear transplantation using two mated dark axolotls, each heterozygous for white and albinism. Shows the following process: fertilized eggs are collected, their cells divide and are then disaggregated into single cells; nuclei from these are selected for donor transplant, drawn from a random blastula; unfertilized white axolotl eggs are activated by electric shock and their nuclei destroyed by ultraviolet irradiation; then a donor-cell nucleus is carefully injected into each of a number of eggs which divide as usual. Observes that the resulting embryos eventually form into a clone of dark larvae. , Reveals, through photomicrography and time-lapse sequences, the research technique of nuclear transplantation using two mated dark axolotls, each heterozygous for white and albinism. Shows the following process: fertilized eggs are collected, their cells divide and are then disaggregated into single cells; nuclei from these are selected for donor transplant, drawn from a random blastula; unfertilized white axolotl eggs are activated by electric shock and their nuclei destroyed by ultraviolet irradiation; then a donor-cell nucleus is carefully injected into each of a number of eggs which divide as usual. Observes that the resulting embryos eventually form into a clone of dark larvae.